Protocol6 min
Optimising annealing temperature with a gradient cycler
A one-run method for finding the annealing temperature that maximises specific product and suppresses primer-dimer.
Read the protocolResources
Written for people at the bench
Protocols, selection guides and technical notes from the applications team. No gated downloads, no lead-capture forms — just the information that makes the method work.
Guide
Choosing between SYBR Green and probe-based qPCR
Cost, multiplexing, and specificity trade-offs — and the melt-curve check that tells you whether SYBR is safe for your assay.
8 min readGuide
Selecting an HPLC column for a new method
How stationary phase, particle size, and pore size interact, and when moving to sub-2 µm actually pays for itself.
9 min readProtocol
Reducing Western blot background
A systematic checklist covering blocking agent, antibody dilution, wash stringency, and membrane choice.
7 min readNote
Cold chain: what actually happens to your enzymes in transit
Why we ship on dry ice, how long a gel pack really holds, and what to check the moment a shipment lands.
5 min readGuide
Specifying a biosafety cabinet for your laboratory
Class and type selection, room airflow, exhaust requirements, and the certification you should insist on at handover.
10 min readNote
FBS lot reservation, and why it matters
Serum lot variability can quietly shift your assay. How reservation and lot-testing protect a long study.
4 min readProtocol
Cryopreservation and post-thaw recovery
Controlled-rate freezing, DMSO exposure time, and the thaw technique that keeps viability above 90 %.
6 min readStuck on a method?
The applications team troubleshoots protocols whether or not the reagent came from us. Describe what is going wrong and someone who has run it will reply.