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AGBiotechLIFE SCIENCE SOLUTIONS
03Express, purify, detect, quantify

Protein Biology & Immunology

The full protein workflow — extraction and purification through electrophoresis, blotting, and immunoassay. Antibodies are validated by application and supplied with lot-specific validation data.

What's in this division

Product range

A representative view. If the item you need is not listed, it can almost certainly still be sourced — ask.

Extraction & Purification

  • RIPA & native lysis buffers
  • Protease & phosphatase inhibitor cocktails
  • Ni-NTA and glutathione resins
  • Protein A/G affinity resins
  • Desalting & concentrating columns
  • Dialysis cassettes & membranes

Quantitation & Assays

  • BCA protein assay kits
  • Bradford reagent
  • Fluorometric protein quantitation
  • Enzyme activity assay kits

Electrophoresis & Blotting

  • Precast and hand-cast SDS-PAGE gels
  • Acrylamide solutions & TEMED
  • Running and transfer buffers
  • PVDF and nitrocellulose membranes
  • Blocking buffers
  • ECL chemiluminescent substrates

Antibodies

  • Primary monoclonal antibodies
  • Primary polyclonal antibodies
  • HRP & AP-conjugated secondaries
  • Fluorophore-conjugated secondaries
  • Loading control antibodies
  • Isotype controls

Immunoassays

  • Sandwich ELISA kits (cytokines)
  • Competitive ELISA kits
  • ELISA coating & wash buffers
  • TMB and OPD substrates
  • Flow cytometry antibody panels

Featured products

Representative line items

Expand any card for full specifications. Every product is supplied against a written quotation.

AG-PB-1002.webp

AG-PB-1002

RIPA Lysis & Extraction Buffer

Balanced ionic and non-ionic detergent buffer that solubilises cytoplasmic, membrane, and nuclear proteins in a single step while preserving immunoreactivity for downstream blotting.

LysisExtractionWestern blot
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AG-PB-2200.webp

AG-PB-2200Best seller

BCA Protein Assay Kit

Colorimetric detection of total protein with high detergent tolerance and a broad working range. Includes BSA standards for a full standard curve.

QuantitationColorimetricBCA
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AG-PB-3050.webp

AG-PB-3050

ClarityECL Chemiluminescent Substrate

Enhanced luminol substrate for HRP-conjugated antibodies, delivering picogram sensitivity with a signal plateau long enough for multi-exposure imaging.

Western blotECLHRP
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AG-PB-4310.webp

AG-PB-4310

Prestained Protein Ladder, 10–250 kDa

Ten recombinant proteins covalently coupled to a chromophore, giving sharp bands for both molecular-weight sizing and visual confirmation of transfer efficiency.

SDS-PAGELadderPrestained
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AG-PB-5120.webp

AG-PB-5120Cold chain

Human IL-6 Sandwich ELISA Kit

Pre-coated 96-well strip plate with matched antibody pair, recombinant standard, and TMB substrate. Validated in serum, plasma, and cell culture supernatant.

ELISACytokineImmunoassay
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AG-PB-6001.webp

AG-PB-6001

Ni-NTA Agarose Affinity Resin

High-capacity nickel-charged agarose for purification of His-tagged recombinant proteins under native or denaturing conditions. Regenerable across multiple cycles.

PurificationHis-tagIMAC
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Applications

What this division is used for

Western blotting & immunodetection
Recombinant protein purification
Cytokine & biomarker quantification
Immunoprecipitation
Flow cytometry phenotyping